MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Urokinase is a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation.
A specific polymorphism in the Urokinase gene may be associated with late onset Alzheimer disease and also with decreased affinity for fibrin binding.
This protein converts plasminogen to plasmin by specific cleavage of an Arg Val bond in plasminogen.
The proprotein is cleaved at a Lys Ile bond by plasmin to form a two chain derivative in which a single disulfide bond connects the amino terminal A chain to the catalytically active, carboxy terminal B chain.
This two chain derivative is also called HMW uPA (high molecular weight uPA).
HMW uPA can be further processed into LMW uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino terminal fragment.
LMW uPA is proteolytically active but does not bind to the uPA receptor.
Applications:Suitable for use in FLISA and Western Blot.
Other applications not tested.
Recommended Dilution:FLISA: 1:10,000Western Blot: 1:5,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.05M sodium phosphate, 0.1M sodium chloride, 1mM EDTA, pH 6.6. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes high molecular weight and low molecular weight mouse Urokinase.
Isotype：IgG
Calc Applications Abbrev：FLISA WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to mouse Urokinase.
