MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus.
Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans.
Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb).
The first step in coronavirus infection is binding of the viral spike protein, a 139-kD protein, to certain receptors on host cells.
The spike protein is the main surface antigen of the coronavirus.
The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.
Applications:Suitable for use in FLISA.
Other applications have not been tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
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Size:100ul
Host:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity:Recognizes the C-terminal of the spike protein of SARS-associated coronavirus.
Isotype:IgG
Calc Applications Abbrev:FLISA
Immunogen:Synthetic peptide corresponding to amino acids at the C-terminus of the SARS Spike glycoprotein.