MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Plasminogen Activator Inhibitor 1 (PAI-1) is a glycoprotein found in plasma that is implicated in the processes of angiogenesis, hemostasis, tumor metastasis, fibrionlysis, ovulation, embryonic development and inflammation.
The elevation of PAI-1 has shown to correlate with the prognosis of many cancers including breast cancer.
PAI-1 is activated by the glycoprotein vitronectin.
Vitronectin binds to PAI-1 and modulates fibrinolysis and cell migration.
This binding has proven to be an important inhibitor of the activated form of protein C (APC).
Applications:Suitable for use in FLISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 1:50,000Western Blot: 1:5,000Immunohistochemistry: 1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.05M sodium phosphate, 0.1M sodium chloride, 1mM EDTA, pH 6.6. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes human PAI-1 both alone and in complex under reducing and native conditions.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human PAI-1.
