MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is further subjected to absorption with immobilized total human serum proteins in order to remove non-specific antibodies.
Next, the antisera fraction is absorbed with immobilized collagen types I, II, IV and V to remove crossreactive antibodies to antigenic determinants common for various collagen types.
The affinity purified antibody is obtained by binding to immobilized native human collagen type III (the antigen used for immunization), followed by elution with acidic buffer, neutralization, dialysis, dispensing and lyophilization.
Specificity was ascertained by competition FLISA.
Complete inhibition is found if the antibody was preincubated with collagen type III.
Inhibition by other types of collagens is observed only at 20-50 times higher concentration.
Reconstitute with 1.0ml distilled water and add preservative if preferred.
Applications: This antibody can be used for immunohistochemistry on frozen cells and sections.
Suitable for dot-blotting and FLISA on native human collagen type III.
Use on paraffin sections is not tested.
May be diluted at least 1:20 for immunohistochemical procedures if Peroxidase labeled secondary antibodies are applied.
If a FITC-labeled secondary antibody is used the antibody can be diluted 1:10.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by ammonium sulfate precipitation and cross-absorption on immobilized other collagen types and affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes Collagen Type III.
Isotype：IgG
Calc Applications Abbrev：FL IHC N WB
Calc Crossreactivity：Hu
Immunogen：Extensively purified native collagen type III extracted from human skin in dilute acidic buffer after mild pepsin digestion.
