84-3207-91 [取扱停止]Fibroblast Growth Factor, Basic (FGFb) (MaxLight 750) 100ul F4210-11A-ML750
特徴
MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Absorbance (759nm); Emission (780nm); Extinction Coefficient 240,000.
bFGF (FGF-2) is a member of a family of seven peptides that are potent regulators of cell proliferation, differentiation and function.
bFGF stimulates the proliferation of cells of mesodermal origin and many cells of endodermal, ectodermal and neuroectodermal origin.
bFGF also induces neuron differentiation, survival and regeneration and stimulates astrocyte migration.
bFGF may also be involved in the production of a variety of pathological conditions arising from excessive cell proliferation.
Applications:Suitable for use in FLISA, Western Blot and Neutralization.
Other applications not tesed.
Recommended Dilutions:FLISA: >0.5ug/ml 100ul/well Allows detection of 0.2–0.4ng/well
Western Blot: 0.1–0.2ug/m Detection limit is 1.5–3.0ng/lane, under either reducing or non-reducing conditions.
Neutralization: 0.46-0.9ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity:Recognizes human FGF-basic.
Isotype:IgG
Calc Applications Abbrev:FLISA N WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to 154aa from human Fibroblast Growth Factor, Basic, expressed in E. coli.
Shelf Life:1year
EU Commodity Code:30021010
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