MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule.
In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex.
In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes.
However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern.
Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma.
Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.
Applications:Suitable for use in Western Blot, Flow Cytometry (Not Tested), Immunofluorescence, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 1-2ug/ml/30min @ RTFlow Cytometry (Not Tested): 0.5-1ug/10e6 cellsImmunofluorescence: 1-2ug/mlImmunoprecipitation: 1-2ug/500ug protein lysateOptimal dilutions to be determined by the researcher.
Positive Control:HeLa or MCF-7 cells.
Breast carcinoma
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity：Recognizes human beta-catenin (p120). Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IF IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to the middle of human beta-Catenin (p120).
