MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors.
VEGFB has structural similarities to VEGF and PIGF and is frequently co-expressed with VEGF.
There are two alternatively spliced isoforms of VEGFB: VEGFB 167 and VEGFB 186.
VEGFB 167, a highly basic heparin-binding protein, remains with the cell or extracellular matrix whereas, VEGFB 186 is readily secreted.
VEGFB stimulates endothelial cell proliferation.
VEGFB binds to the tyrosine kinase receptor VEGFR1 (flt1) and does not bind to VEGFR2.
Vascular Endothelial Growth Factor B is widely expressed but is most abundant in heart, skeletal muscle, and pancreas.
It has been suggested that VEGFB expression in human primary breast cancers is associated with lymph node metastasis.
The genes that encode VEGFB have been mapped to chromosome 11q13.
Applications:Suitable for use in Western Blot, Immunohistochemistry, FLISA
Recommended Dilution:FLISA1:1,000 Western Blot1:50-100Immunohistochemistry: 1:10-50
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu
Immunogen：VEGFB (VEGF2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117~147 amino acids from the Center region of human VEGF2.
