MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
The metaphase-to-anaphase transition is the final discrete event in duplication and separation of the genetic material of the cell.
Its timing is regulated by the activation of the anaphase-promoting complex (APC).
In both budding and fission yeast, the degradation of the Pds1 or Cut2 protein, respectively, is required for the onset of sister chromatid separation.
Both proteins are APC substrates.
Pds1 and Cut2 proteins associate with the yeast separin proteins Esp1 and Cut1, respectively, and prevent the separins from promoting chromatid separation.
Pds1 and Cut2 are also called anaphase inhibitors or securins
Applications:Suitable for use in FLISA, Dot Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Immunohistochemistry: 1:50-1:100Dot Blot: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity：Recognizes human SEPARIN when phosphorylated at Ser1126.
Isotype：IgG
Calc Applications Abbrev：DB FLISA IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser1126 of human SEPARIN (KLH).
