84-3076-52 [取扱停止]CREB, phosphorylated (Ser133) (cAMP Response Element-binding Protein, CREB1) (MaxLight 650) 100ul C7915-05H-ML650
特徴
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
CREB is a transcription factor that is a member of the leucine zipper family of DNA binding proteins.
This protein binds as a homodimer to the cAMP-responsive element, (CRE), a sequence present in many viral and cellular promoters.
The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.
Applications:Suitable for use in FLISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity:Recognizes human CREB phosphorylated at Ser133.
Isotype:IgG
Calc Applications Abbrev:FLISA IHC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser133 of human CREB (KLH).
Shelf Life:1year
Swiss Prot Number:P16220
EU Commodity Code:30021010
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