MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
The covalent attachment of prenyl lipids, such as farnesyl or geranylgeranyl groups, by specific transferases is indispensable for the cellular sorting of many proteins.
Peroxisomal farnesylated protein or PXF, which localizes to the outer surface of peroxisomes, is 93% identical in sequence to human HK33, a predicted 299 amino acid protein.
The ubiquitous transcription of the HK33 gene indicates its role as a housekeeping gene.
At least four variants of HK33 have been identified, two of which are farnesylated in vitro.
Evidence suggests that PXF is involved in the process of peroxisomal biogenesis or assembly.
Complementation group J (CG-J) from patients with a peroxisome biogenesis disorder (PBD), such as Zellweger syndrome, has been identified.
Human PXF expression restored peroxisomal protein import in fibroblasts from a patient with Zellweger syndrome of CG-J.
This patient was homozygous for a point mutation in PXF.
These results indicate that PXF is the causative gene for CG-J PBD and suggests that the C-terminal part of the PXF protein, including the CAAX homology box, is required for function.
Applications:Suitable for use in FLISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity：Recognizes human PXF.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide selected from the C-terminal region of human PXF (KLH).
