MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
Ubiquilin 1 (UBQLN1), also known as DA41, was isolated from an adult rat lung cDNA library, and encodes a cellular protein that associates with DAN.1 DAN expression is reduced in rat fibroblast 3Y1 cells transformed with mouse sarcoma virus and in rodent fibroblasts transformed with a variety of oncogenes.
The DAN-DA41 interaction is mediated through the N-terminal domain and a cysteine-knot region of DAN.
Human DA41 encodes a 589aa protein with 86% aa sequence identity with rat protein.2 DA41 expression is regulated in a cell cycle-dependent manner.
PLIC1 and PLIC2 (UBQLN2) are homologs of the mouse Plics (proteins linking integrin-associated protein (IAP) and cytoskeleton) and the yeast Dsk2 protein.
PLIC1, also called UBQLN1, shares 72% aa identity with PLIC2,3 Two motifs are conserved in the mammalian PLICs and yeast Dsk2, an N-terminal ubiquitin-like (UBL) domain and a C-terminal ubiquitin-associated (UBA) domain.
Unlike ubiquitin, the UBL domain of the PLICs does not have a diglycine motif in its C terminus.
The UBA domain is present in multiple enzyme classes of the ubiquitination machinery.
Human PLICs associate with both proteasomes and ubiquitin ligases in large complexes.
Overexpression of PLICs impairs the in vivo degradation of 2 unrelated ubiquitin-dependent proteasome substrates, p53 and I-kappa-B-alpha (NFKBIA), but not a ubiquitin-independent substrate.
PLICs may link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.
The DA41 gene maps to chromosome 9q21.2-q21.3, a position overlapping a candidate tumor suppressor locus for bladder cancer.2
Applications:Suitable for use in FLISA and Western Blot.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity：Recognizes human Ubiquilin1. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：FLISA WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide selected from the N-terminal region of human Ubiquilin1 (KLH).
