MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
Higher animals establish host defense by orchestrating innate and adaptive immunity.
This is mediated by professional antigen presenting cells, i.e.
dendritic cells (DCs).
DCs can incorporate pathogens, produce a variety of cytokines, maturate, and present pathogen-derived peptides to T cells, thereby inducing T cell activation and differentiation.
These responses are triggered by microbial recognition through type I transmembrane proteins, Toll-like receptors (TLRs) on DCs.
TLRs consist of ten members and each TLR is involved in recognizing a variety of microorganism-derived molecular structures.
TLR ligands include cell wall components, proteins, nucleic acids, and synthetic chemical compounds, all of which can activate DCs as immune adjuvants.
Each TLR can activate DCs in a similar, but distinct manner.
For example, TLRs can be divided into subgroups according to their type I interferon (IFN) inducing ability.
TLR2 cannot induce IFN-alpha or IFN-beta, but TLR4 can lead to IFN-beta production.
Meanwhile, TLR3, TLR7, and TLR9 can induce both IFN-alpha and IFN-beta.
Recent evidences suggest that cytoplamic adapters for TLRs are especially crucial for this functional heterogeneity.
Applications:Suitable for use in FLISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity：Recognizes mouse TLR1.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide selected from the C-terminal region of mouse TLR1 (KLH).
