MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
p40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application.
The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors.
The ability to differentiate between lung adenocarcinoma vs.
squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment.
p63 antibody's ability to distinguish between the tumor types appears to be inferior when compared to p40.
The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.
Applications:Suitable for use in Flow Cytometry (Not Tested), Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (FFPE): Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutesOptimal dilutions to be determined by the researcher.
Positive Control:HEK293 cells or Prostate Carcinoma or Lung Squamous Cell Carcinoma
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes human p40 (p63 Delta). Species Crossreactivity: mouse, rat, bovine
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa5-17 (ENNAQTQFSEPQY) from human p40 (p63 Delta).
