MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends.
Prenylation was initially believed to be important only for membrane attachment.
However, another role for prenylation appears to be its importance in protein-protein interactions.
The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins.
Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif.
This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A.
The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain.
It may be a component of a prelamin A endoprotease complex.
The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina.
It shares limited sequence similarity with iron-only bacterial hydrogenases.
Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
Applications:Suitable for use in Western Blot, FLISA
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：FLISA WB
Calc Crossreactivity：Hu
Immunogen：NARF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-160 amino acids from the Central region of human NARF.
