MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family.
Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions.
In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins.
Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin.
VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility.
VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions.
VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Applications:Suitable for use in Western Blot, Immunohistochemistry and FLISA.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry: Formalin-fixed, paraffin-embedded sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes human VASP.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 267-296 in the C-terminal region of human VASP, UniProt Accession #P50552.
