MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
This gene encodes a member of the epidermal growth factor superfamily.
The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide.
This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types.
This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor.
Defects in this gene are the cause of hypomagnesemia type 4.
Dysregulation of this gene has been associated with the growth and progression of certain cancers.
Alternate splicing results in multiple transcript variants.
Applications:Suitable for use in Western Blot, Immunohistochemistry and FLISA.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Formalin fixed and paraffin embedded tissues.
Heat induced antigen retrieval with citrate buffer, pH 6.0 is recommended
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A and peptide affinity chromatography
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity:Recognizes human EGF. Species Crossreactivity: mouse
Isotype:IgG
Calc Applications Abbrev:FLISA IHC WB
Calc Crossreactivity:Hu Mo
Immunogen:KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 690-720 in the central region of human EGF