MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts.
AKT1 and the related AKT2 are activated by platelet-derived growth factor.
The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1.
It was shown that the activation occurs through phosphatidylinositol 3-kinase.
In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival.
Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Multiple alternatively spliced transcript variants have been found for this gene.
[provided by RefSeq].
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry (Not Tested) and FLISA.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes human AKT1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：FLISA IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 428-457 in the C-terminal region of human AKT1, UniProt Accession #P31749. Species Sequence Homology: rat and bovine
