84-2953-69 [取扱停止]V5 Tag (MaxLight 550) 100ul 043700-ML550
特徴
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
V5 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope.
The V5 epitope tag is derived from an epitope present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
Usually the V5 tag is used with all 14aa (GKPIPNPLLGLDST), although it has also been used with a shorter 9aa sequence (IPNPLLGLD).
Applications:Suitable for use in Western Blot and FLISA.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Grade:Purified
Purity:Purified by ammonium sulfate precipitation
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity:Recognizes V5 Tag.
Isotype:IgG
Calc Applications Abbrev:FLISA WB
Immunogen:KLH conjugated synthetic peptide encoding the V5 tag, GKPIPNPLLGLDST.
Shelf Life:1year
EU Commodity Code:30021010
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