MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
TP73 is tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development.
The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis.
The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters.
The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors.
Applications:Suitable for use in FLISA, Western Blot, Immunohistochemistry Flow Cytometry (Not Tested) and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunohistochemistry (FFPE): 1:50-1:100Flow Cytometry (Not Tested): 1:10-1:50Immunofluorescence: 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity:Recognizes human TP73. Species Crossreactivity: mouse
Isotype:IgG
Calc Applications Abbrev:FLISA IF IHC WB
Calc Crossreactivity:Hu Mo
Immunogen:KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 288-317 in the central region of human TP73, UniProt Accession #O15350