MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily.
Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts.
The encoded protein acts as a nuclear transcription factor.
Translocation of the protein from the nucleus to mitochondria induces apoptosis.
Applications:Suitable for use in FLISA and Dot Blot.
Other applications not tested.
Recommended Dilution:Dot Blot: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes human NR4A1 when phosphorylated at Ser351.
Isotype：IgG
Calc Applications Abbrev：DB FLISA
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic phosphopeptide mapping to a fragment of amino acid residues surrounding S351 of human NR4A1, UniProt Accession #P22736. Species sequence homology: mouse, rat
