84-2947-21　［取扱停止］MyoGEF, NT (Myosin-interacting Guanine Nucleotide Exchange Factor, FLJ10665, MGC126315, MGC126353, MGC126354, Pleckstrin Homology Domain-containing Family G Member 6, PH Domain-containing Family G Member 6, PLEKHG6) (MaxLight 550) 100ul　M9789-85-ML550

特徴

• MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
• Guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation.
• It Also activates RHOG and does not activate RAC1, or to a much lower extent than RHOA and RHOG.
• Its part of a functional unit, involving PLEKHG6, MYH10 and RHOA, at the cleavage furrow to advance furrow ingression during cytokinesis.
• In epithelial cells, It is required for the formation of microvilli and membrane ruffles on the apical pole.
• Along with EZR, it is required for normal macropinocytosis.
• Applications:Suitable for use in FLISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:FLISA: 1:1,000Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™550 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.