84-2944-97　［取扱停止］Hexokinase 2 (Hexokinase-2, Hexokinase Type II, HK II, HKII, HK2, HXK2, DKFZp686M1669, Muscle Form Hexokinase) (MaxLight 550) 100ul　H2035-30F-ML550

特徴

• MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
• In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV.
• Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P.
• Hexokinase activity is involved in the first step in several metabolic pathways.
• HK3 is bound to the outer mitochondrial membrane.
• Its hydrophobic N-terminal sequence may be involved in membrane bindng.
• It is the predominant hexokinase isozyme expressed in insuline-responsive tissues such as skeletal muscle.
• The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other.
• The catalytic activity is associated with the C-terminus while regulatory function is associated wiht the N-terminus.
• Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.
• Applications:Suitable for use in FLISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™550 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.