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試薬
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US Biological(antibodypab)
84-2936-91 [取扱停止]Ribophorin, CT (Dolichyl-diphosphooligosaccharide-Protein Glycosyltransferase 67kD Subunit, Dolichyl-diphosphooligosaccharide-Protein Glycosyltransferase Subunit 1, Ribophorin I, Ribophorin-1, RPN-I, RPN1) (MaxLight 550) 100ul R2031-16A-ML550
84-2936-91 [取扱停止]Ribophorin, CT (Dolichyl-diphosphooligosaccharide-Protein Glycosyltransferase 67kD Subunit, Dolichyl-diphosphooligosaccharide-Protein Glycosyltransferase Subunit 1, Ribophorin I, Ribophorin-1, RPN-I, RPN1) (MaxLight 550) 100ul R2031-16A-ML550
特徴
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Ribophorins 1 and 2 are abundant and highly conserved glycoproteins residing in the endoplasic reticulum, that participate in ribosome binding.
Mammalian oligosaccharyltransferase activity is associated with a protein complex composed of RPN1, RPN2, and an oligosaccharyltransferase protein.
RPN1 is a component of the proteasome base.
The ubiquitin-like (UBL) domain of recombinant Rad23 interacts with proteasomes through the leucine-rich repeat domain of RPN1.
The RPN1 gene maps to chromosome 3 in somatic cell hybrids, and the RPN2 gene maps to chromosome 20 by in situ hybridization.
Applications:Suitable for use in FLISA and Western Blot.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity:Recognizes human RPN1. Species Crossreactivity: mouse.
Isotype:IgG
Calc Applications Abbrev:FLISA WB
Calc Crossreactivity:Hu Mo
Immunogen:Synthetic peptide selected from the C-terminal region of human RPN1 (KLH).
Shelf Life:1year
Swiss Prot Number:P04843
EU Commodity Code:30021010
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