84-2935-91 [取扱停止]Ubiquitin Activating Enzyme E1, CT (A1S9 Protein, UBE1, Ubiquitin-like Modifier-activating Enzyme 1, UBA1, A1S9T, UBE1) (MaxLight 550) 100ul U1000-08A-ML550
特徴
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation.
This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair.
It is part of a gene cluster on chromosome Xp11.23.
Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR.
Applications:Suitable for use in FLISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity:Recognizes human UBE1. Species Crossreactivity: mouse.
Isotype:IgG
Calc Applications Abbrev:FLISA IHC WB
Calc Crossreactivity:Hu Mo
Immunogen:Synthetic peptide selected from the C-terminal region of human UBE1 (KLH).
Shelf Life:1year
EU Commodity Code:30021010
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