84-2935-22 [取扱停止]EIF4B (S422) (Eukaryotic Initiation Factor 4B, Eukaryotic Translation Initiation Factor 4B, hCG_2014609, eIF4B) (MaxLight 550) 100ul E8949-39J-ML550
特徴
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
EIF4B is required for the binding of mRNA to ribosomes.
It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP.
It also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F
Applications:Suitable for use in FLISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Western Blot: 1:50-1:100Immunohistochemistry: 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity:Recognizes human EIF4B.
Isotype:IgG
Calc Applications Abbrev:FLISA IHC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to amino acid residues surrounding S422 of human EIF4B (KLH).
Shelf Life:1year
Swiss Prot Number:Q8WYK5
EU Commodity Code:30021010
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