84-2933-45　［取扱停止］PUMA, BH3 Domain (Bcl-2-binding Component 3, p53 Upregulated Modulator of Apoptosis, JFY-1, BBC3) (MaxLight 550) 100ul　P9198-02N-ML550

特徴

• MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
• PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53.
• The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b.
• PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53.
• PUMA may be a direct mediator of p53-induced apoptosis.
• Applications:Suitable for use in FLISA, Western Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:FLISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™550 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.