MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
CNK, a member of the CDC5/Polo subfamily of Ser/Thr protein kinases, is involved in regulating M phase functions during the cell cycle.
It may also be part of the signaling network controlling cellular adhesion.
In vitro, is able to phosphorylate CDC25C and casein.
This membrane-associated protein binds to the calcium/integrin-binding protein (CIB).
This interaction probably occurs via the POLO-box domain.
Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain.
This protein exhibits a short half-live.
In cells of hematopoietic origin, CNK is strongly and exclusively detected in terminally differentiated macrophages.
Transcript expression appears to be down-regulated in primary lung tumor.
Cytokine and cellular adhesion trigger CNK induction.
CNK is thought to be phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis The protein contains 2 POLO box domains.
Applications:Suitable for use in Western Blot, FLISA
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Human, mouse
Isotype：IgG
Calc Applications Abbrev：FLISA WB
Calc Crossreactivity：Hu Mo
Immunogen：PLK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 617~646 amino acids from the C-terminal region of human PLK3.
