MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
SCYL1 forms multimers following transfection into COS-7 cells.
SCYL1 forms a 300-kD trimer using crosslinking reagents.
Biochemical analysis revealed no phosphorylation or autophosphorylation activity.The 707-amino acid SCYL1 variant, variant 2, localized to centrosomes during mitosis.
During interphase, fluorescence-tagged variant 2 localized in the cytoplasm as well as centrosomes.
However, at the beginning of mitosis, the fluorescence appeared as a pair of bright nuclear foci that followed centrosome localization throughout mitosis, while maintaining diffuse cytoplasmic labeling.
Endogenous variant 2 in HeLa cells showed a similar staining pattern.
Centrosomal localization was independent of microtubules.
Applications:Suitable for use in Western Blot, Immunohistochemistry, FLISA
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu
Immunogen：SCYL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SCYL1.
