84-2884-76　［取扱停止］F13B, NT (F13B, Coagulation factor XIII B chain, Fibrin-stabilizing factor B subunit, Protein-glutamine gamma-glutamyltransferase B chain, Transglutaminase B chain) (MaxLight 490) 100ul　035305-ML490

特徴

• MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
• This gene encodes coagulation factor XIII B subunit.
• Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade.
• Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits.
• The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as a plasma carrier molecules.
• Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin.
• Upon activation by the cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII.
• This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot.
• Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone.
• These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion.
• Applications:Suitable for use in Western Blot, FLISA
• Recommended Dilution:FLISA: 1:1,000Western Blot: 1:100-500
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™490 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.