MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP.
The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage.
The enzyme is used as a marker of lymphocyte differentiation.
Consequently, a deficiency of NT5E occurs in a variety of immunodeficiency diseases.
Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.
It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.
Applications:Suitable for use in Western Blot, Immunohistochemistry and FLISA.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry: Formalin fixed and paraffin embedded tissuesOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes human CD73. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 520-550 in the C-terminal region of human CD73, UniProt Accession #P21589.
