84-2883-67　［取扱停止］PPM1F, ID (PPM1F, KIAA0015, POPX2, Protein phosphatase 1F, Ca(2+)/calmodulin-dependent protein kinase phosphatase, Partner of PIX 2, Protein fem-2 homolog) (MaxLight 490) 100ul　040325-ML490

特徴

• MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
• The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases.
• PP2C family members are known to be negative regulators of cell stress response pathways.
• This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases.
• Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase.
• The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis.
• An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.
• Applications:Suitable for use in Western Blot and FLISA.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™490 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.