MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle.
Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site.
The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription.
In addition, this protein regulates alternate splicing of the CFTR gene.
A similar pseudogene is pr[sent on chromosome 20.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and FLISA.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry: Formalin-fixed, paraffin-embedded sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes human TARDBP at ~45kD. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：FLISA IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa1-30 in the N-terminal region of human TARDBP, Swiss/UniProt Accession: Q13148 Cellular Localization: Nucleus. Species Sequence Homology: chicken
