MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK).
MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK.
The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells.
MEKK1 can phosphorylate and activate MAPKK 1 and MAPKK 2 (MEK1/MEK2) which leads to phosphorylation of MAP kinases.
It is also a highly efficient activator of the JNK cascade.
The protein contains a putative 1 RING-type zinc finger and 1 SWIM-type zinc finger.
Applications:Suitable for use in FLISA and Western Blot.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes human MEKK1.
Isotype：IgG
Calc Applications Abbrev：FLISA WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide selected from the C-terminal region of human MEKK1 (KLH).
