84-2808-41　［取扱停止］MARK4, NT (MAP/microtubule Affinity-regulating Kinase 4, KIAA1860, MAP/microtubule Affinity-regulating Kinase-like 1, MARKL1, MARKL1L, MARK4L, MARK4S) (MaxLight 490) 100ul　M2408-01C-ML490

特徴

• MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
• MARK4 contains an N-terminal serine/threonine kinase domain, a central ubiquitin-associated domain, and a C-terminal KA1-associated kinase domain.
• RT-PCR analysis detects upregulated expression of the gene for MARK4 in nearly all clinical hepatocellular carcinoma cells.
• Northern blot analysis reveals ubiquitous expression of a 3.6-kb transcript, with highest expression in testis.
• Immunofluorescence microscopy demonstrates homogeneous cytoplasmic expression.
• Colony-forming assays show that MARK4 antisense reduces the growth of SNU475 cells.
• It has been suggested that MARK4 provides a growth advantage to cells.
• Applications:Suitable for use in FLISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:FLISA: 1:1,000Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™490 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.