84-2807-07　［取扱停止］HLA Class 1 Antigen G (HLA Cass I Histocompatibility Antigen alpha Chain G, HLA G Antigen, HLA G, HLAG, HLA-G, HLA-6.0, Major Histocompatibility Complex Class I G, MHC-G) (MaxLight 490) 100ul　H6098-72V-ML490

特徴

• MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
• HLA-G belongs to the HLA class I heavy chain paralogues.
• This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin).
• The heavy chain is anchored in the membrane.
• HLA-G is expressed on fetal derived placental cells.
• The heavy chain is approximately 45kD and its gene contains 8 exons.
• Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and FLISA.
• Other potential application, though not tested is Direct Flow Cytometry.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry: Formalin fixed and paraffin embedded tissues .
• Requires HIER with EDTA buffer, pH 9.0Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™490 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.