MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
HCK is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types.
The encoded protein may help couple the Fc receptor to the activation of the respiratory burst.
In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils.
Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.
Applications:Suitable for use in FLISA and Western Blot.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000 Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity:Recognizes human HCK.
Isotype:IgG
Calc Applications Abbrev:FLISA WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide selected from the N-terminal region of human HCK (KLH).