84-2804-81 [取扱停止]DIO1, NT (Type I Iodothyronine Deiodinase, Type-I 5'-deiodinase, Type 1 DI, DIOI, 5DI, TXDI1, ITDI1) (MaxLight 490) 100ul D1080-15-ML490
特徴
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
DIO1 is a thiol-requiring propylthiouracil-sensitive oxidoreductase.
It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3).
It also degrades both hormones by inner ring deiodination (IRD).
Applications:Suitable for use in FLISA and Western Blot.
Other applications not tested.
Recommended Dilutions:FLISA: 1:1000Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A and peptide affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity:Recognizes human DIO1 at ~28kD.
Isotype:IgG
Calc Applications Abbrev:FLISA WB
Calc Crossreactivity:Hu
Immunogen:KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa36-66 of the N-terminal region of human DIO1.
Shelf Life:1year
Swiss Prot Number:P49895
EU Commodity Code:30021010
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