MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
CIT is a putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1.
It probably binds p21 with a tighter specificity in vivo.
This protein exhibits dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues.
CIT plays an important role in the regulation of cytokinesis and the development of the central nervous system.
Applications:Suitable for use in FLISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity:Recognizes human CIT.
Isotype:IgG
Calc Applications Abbrev:FLISA IHC
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide selected from the C-terminal region of human CIT (KLH).