MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
ACE2 cDNA encodes a deduced 805aa protein containing a potential 17aa N-terminal signal peptide and a putative 22aa C-terminal membrane anchor.
It also possesses a zinc metalloprotease consensus sequence and a conserved glutamine residue that may function as a third zinc ligand.
ACE2 is expressed predominantly in vascular endothelial cells of the heart and kidney.
ACE converts angiotensin I to angiotensin II, ACE2 converts angiotensin I to angiotensin 1-9, which has 9aa.
Angiotensin II is a potent blood vessel constrictor, while angiotensin 1-9 does not impact blood vessels but is cleaved by ACE to a shorter peptide, angiotensin 1-7, which is a blood vessel dilator.
Spike (S) proteins of coronaviruses, including the SARS coronavirus, bind with cellular receptors to mediate infection of target cells.
ACE2 binds the S1 domain of the SARS coronavirus S protein.
SARS coronavirus replicates efficiently on ACE2-transfected but not mock-transfected 293T cells.
Anti-ACE2 but not anti-ACE1 antibody blocks viral replication on Vero E6 cells.
It has been proposed that ACE2 is a functional receptor for SARS coronavirus.
Applications:Suitable for use in FLISA, Western Blot and Immunohistochemisty.
Other potential application, though not tested is Direct Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes human ACE2.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 140-172 in the central region of human ACE2.
