MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
ATF2 is a transcription factor that is a member of the leucine zipper family of DNA binding proteins.
This protein binds to the cAMP-responsive element (CRE), an octameric palindrome.
The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription.
The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components.
Applications:Suitable for use in FLISA and Dot Blot.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000 Dot Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes human ATF2 when phosphorylated at Ser322.
Isotype：IgG
Calc Applications Abbrev：DB FLISA WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser322 of human ATF2 (KLH).
