MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Rab5 is required for the fusion of plasma membranes and early endosomes.
Applications:Suitable for use in FLISA, Western Blot, Immunofluorescence, Flow Cytometry (Not Tested) and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:FLISA: 1:1000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Immunofluorescence: 1:10-1:50Flow Cytometry (Not Tested): 1:10- 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes human Rab5.
Isotype：IgG
Calc Applications Abbrev：FLISA IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to a region near corresponding to a portion of amino acids within aa186-215 at the C-terminal region of human Rab5, conjugated to KLH.
