MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Cyclin E1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle.
Cyclins function as regulators of CDK kinases.
Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event.
Cyclin E1 forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition.
Cyclin E1 accumulates at the G1-S phase boundary and is degraded as cells progress through S phase.
Overexpression of Cyclin E1 has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.
This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.
Applications:Suitable for use in Immunohistochemistry, Flow Cytrometry and Western Blot.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry (formalin-fixed, parafin-embedded): 1:50-1:100Flow Cytrometry: 1:10-1:50Western Blot: 1:50-1:100Optimal dilutions to be determined by researcher.
Positive Control:A2058 cell line lysates, human testis tissue and NCI-H292 cells.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes human Cyclin E1 at ~47kD.
Isotype：IgG
Calc Applications Abbrev：FC IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the C-terminal region of human Cyclin E1 conjugated to KLH (P24864).
