MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake, thereby mediating the metabolic functions of insulin.
Binding to insulin stimulates association of the receptor with downstream mediators including IRS1 and phosphatidylinositol 3'-kinase (PI3K).
This protein can activate PI3K either directly by binding to the p85 regulatory subunit, or indirectly via IRS1.
After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked.
Applications:Suitable for use in FLISA and Dot Blot.
Other applications not tested.
Recommended Dilutions:Dot Blot: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes human INSR when phosphorylated at Tyr1185.
Isotype：IgG
Calc Applications Abbrev：DB FLISA
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic phosphopeptide mapping to a fragment of residues surrounding Tyr1185 of human INSR, UniProt Accession #P06213. Species Sequence Homology: bovine, mouse, rat, Xenopus and Drosophilia
