MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
The ADP-ribosylation factor (Arf) family are highly conserved members of the Ras superfamily of regulatory GTP-binding proteins.
Arf proteins participate in routing of intracellular proteins to and within the Golgi complex.
Cellular functions include maintenance of organelle integrity, coat protein assembly, as an activator of phospholipase D.
The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins.
The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization.
Applications:Suitable for use in FLISA and Western Blot.
Other applications not tested.
Recommended Dilutions:FLISA: 1:1000 Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A/G affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity:Recognizes human METAP2.
Isotype:IgG
Calc Applications Abbrev:FLISA WB
Calc Crossreactivity:Hu
Immunogen:KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 10-40 in the N-terminal region of human METAP2, UniProt Accession #P50579; NP_006829.