MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells.
In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments.
Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway.
Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.
Applications:Suitable for use in FLISA, Western Blot, Immunofluorescence and Immunohistochemisty.
Other potential application, though not tested is Direct Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Formalin fixed and paraffin embedded tissues.
Heat induced antigen retrieval with citrate buffer, pH 6.0 is recommended
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes human NES
Isotype：IgG
Calc Applications Abbrev：FLISA IF IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1389-1416 of human NES
