84-2793-73　Interleukin 6 (IL-6) (MaxLight 490) 100ul　I8428-25A-ML490

特徴

• MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
• Interleukin-6 (IL-6) is a pleotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses.
• IL-6 is produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes.
• It has diverse biological functions.
• It stimulated B cell differentiation and antibody production.
• It synergizes with IL-3 in megakaryocyte development and platelet production.
• It induces expression of hepatic acute phase proteins.
• It regulated bone metabolism.
• IL-6 signals through the IL-6 receptor system that consists of two chains: IL-6Ra and gp130.
• Applications:Suitable for use in Neutralization, FLISA and Western Blot.
• Other applications have not been tested.
• Recommended Dilution:Neutralization: 0.2-0.4ug/ml yields one-half maximal inhibition [ND50] of the biological activity (1ng/ml)
• Direct FLISA: 0.5ug/ml (100ul/well).
• The detection limit is 0.2-0.4ng/well
• Western Blot: 0.1-0.2ug/ml.
• The detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Note: Applications are based on unconjugated antibody.