MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription.
The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2.
Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone.
Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.
Applications:Suitable for use in Western Blot and Immunofluorescence (IC).
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000Immunofluorescence (IC): 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity：Recognizes human ESR1.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa452-480 in the C-terminal region of human ESR1 isoform1, UniProt Accession #NP_000116.2; P03372 Species sequence homology: mouse, porcine, rat.
