MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell.
TRP-PLIK is a protein that is both an ion channel and a kinase.
As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium.
As a kinase, it is capable of phosphorylating itself and other substrates.
The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.[supplied by OMIM]
Applications:Suitable for use in FLISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity:Recognizes human TRPM7.
Isotype:IgG
Calc Applications Abbrev:FLISA IHC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide selected from the N-terminal region of human TRPM7 (KLH).