MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
PAK5 is a member of the PAK family of Ser/Thr protein kinases.
PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling.
This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP.
This kinase is predominantly expressed in brain.
It is capable of promoting neurite outgrowth, and thus may play a role in neurite development.
This kinase is associated with microtubule networks and induces microtubule stabilization.
The subcellular localization of this kinase is tightly regulated during cell cycle progression.
Applications:Suitable for use in FLISA, Western Blot, Immunoprecipitation, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:10-1:50Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity：Recognizes human PAK5.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide selected from corresponding to a portion of amino acids within aa183-198 of human PAK5 (KLH).
