MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.
This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4).
The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
This gene is intronless and encodes a member of the histone H3 family.
Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Applications:Suitable for use in FLISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity：Recognizes human Histone H3.1 when phosphorylated at Ser10.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to a portion of amino acids within aa residues surrounding Ser10 of human HIST1H3B (MAPS).
